RAPID COMMUNICATION Recombinant Retroviruses Pseudotyped With the Vesicular Stomatitis Virus G Glycoprotein Mediate Both Stable Gene Transfer and Pseudotransduction in Human Peripheral Blood Lymphocytes

It is essential for the study of T-cell function and the improveditions. However, VSV-G–pseudotyped virions may cause ment of adoptive cell therapies to efficiently generate large transduction artifacts that must be carefully excluded. The populations of human primary T cells that reliably express VSV-G virions require 10to 100-fold higher concentrations foreign genes. This goal is achieved by using recombinant of infectious particles to achieve levels of gene transfer comretroviruses pseudotyped with either the gibbon ape leukeparable to GaLV-virions. Nonetheless, the physical stability mia virus (GaLV) envelope or the vesicular stomatitis virus of VSV-G–coated particles enables the concentration of viral G (VSV-G) glycoprotein. We show here that both retroviral stocks to 10 infectious particles per milliliter or more. particles mediate stable gene transfer in CD4 and in CD8 q 1997 by The American Society of Hematology. peripheral blood lymphocytes cultured under optimized con-